Lab Report on TLC analisys of Analgestic Drugs Essay

In this experiment, thin-layer chromatography (tender loving care) was apply to determine the composition of various over-the-counter (OTC) analgesics Anacin, Bufferin, Excedrin, and Tylenol. The TLC places were first viewed under ultraviolet light (UV) light and and then treated with ace vapor in order to cast the snoopting. Experiment SchemeInitially, sixteen hairlike micropipets were created in order to spot the TLC home plates. Two TLC plates were then obtained and attach with pencil for spotting. A line was draw 1 cm from the bottom of severally plate, and five small, evenly spaced mark were make along those lines (see foreshadow 1). for each one mark indicated where a substance would be scratchy.All compounds used were in solutions of 1g of each dissolved in 20 ml of a 5050 potpourri of m ethyl groupene chloride and ethanol. The first plate made was the character reference plate. Capillary micropipets were used to spot the first 4 marks with acetaminophen, as pirin, caffeine, and salicylamide (in that order). (See figures 2-5 for chemical substance structures.)The last mark was spotted with a reference solution of all quad chemicals. The sulphur plate made was the precedent plate. The first four marks were spotted with Anacin, Bufferin, Excedrin, and Tylenol. The fifth mark was spotted with a reference solution of all four drugs. Figure 1. Prepared TLC platesFigure 2. AcetaminophenFigure 3. AspirinFigure 4. CaffeineFigure 5. SalicylamideA cultivation container was created with a wide-mouthed screwcap flap. It was filled with the maturation solvent, which was .5% frozen acetic acid in ethyl acetate, so that the solvent was or so .5 cm deep.The first TLC plate was then carefully laid into the development container. Great care was taken to go out that the plate went in evenly so that the solvent could rise evenly up the plate. Once the solvent prior had reached approximately 1cm from the top of the plate, the plate was removed, t he solvent front was marked with a pencil, and the plate was allowed to dry.The second plate was then placed in the development chamber in the kindred manner as the first. Once the solvent front reached approximately 1cm from the top of the plate, the plate was removed, the solvent front was marked with a pencil, and the plate was allowed to dry. Each plate was then viewed under the UV light.Any vagrant that were seen were lightly circled with a pencil, and their color was noted. The orders of elution (Rf values) were mensurable by dividing the distance from the service line to the gist of the spot by the distance from the baseline to the solvent front. After all observations and calculations were made, the plates were placed in a jar containing iodin.The jar was warmed with hands so that the iodine vaporized. The plates were then removed from the jar and observed. The reference and sample plates were then compared to determine which compounds the drugs on the sample plate contai ned. Data